Microbiology
Microbiology is branch of biology which deals with microorganisms and clinical microbiology deals with microorganisms responsible for diseases.
In laboratory, suspected samples such as pus from wounds or secretions, body fluids such as blood, Cerebrospinal fluids, pleural fluids are inoculated in enrichment media where the microorganism such as bacteria and fungi can grow fast and freely.
After stipulated time, they are inoculated on culture media such as blood agar, nutrient agar, MacKonkey?s agar and further incubated for 24 hrs.
Fig 1. :- Slide - Acid Fast Bacilli Seen
The organisms grow in colonies and then biochemical tests are performed to identify the organisms.
These organisms are then tested for sensitivity test, i.e., they are tested for drugs to which they are responding.
In
automated instruments the major processes like detection and identification are
done by the instruments. Further, the drug sensitivity is also tested by instruments
and that too in much lesser time than manual methods
Fig 2. :- Biomerieux Instrument Bact-Alert
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